Cell Line Identification
Research findings produced from biomedical research performed with cultured cells can become threatened by contamination and misidentification. For these issues, the National Institutes of Health (NIH) has recommended and provided guidelines for STR cell line identification, in order for researchers to receive grants and/or to be published. (click here to view the NIH guidelines)
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August 10, 2010: BioTechniques® Newsletter:
"After 50 years of skepticism, finger pointing, and unenforced protocols, sentiments are growing for mandatory cell line authentication as a condition for funding and publication." ...read more
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DDC is delighted to provide fast, accurate cell line identification services in partnership with Promega Corporation.
UK's Leading DNA Testing Company - Authenticate your cell lines at DDC.
Who Can Benefit
- University Research Programs
- Biomedical Researchers
What We Need
- Purified DNA - 10-1000 μl at a minimum of 2 ng/μl
- or -
- Cell pellet containing 1x106 - 5x106 cells
What You Receive
- STR Allele Report and a Description of Results
- Electropherogram
- Rapid Testing Time-Results in 5 days
StemElite™ ID System & PowerPlex® 16
StemElite™ ID and/or PowerPlex® 16 provide powerful and complete systems for STR cell line identification. The StemElite™ ID system incorporates a sensitive marker that specifically detects the presence of contaminating mouse DNA. Both StemElite™ ID and PowerPlex® 16 use all the standard American Type Culture Collection (ATCC) markers for authenticating cell lines.
DDC's Testing Offers
- Unrivalled quality with 15 years of experience in Human Identification using STR Profiling
- Expert client service
- Laboratory accreditations from AABB, CLIA, ACLASS for ISO/IEC 17025, CAP, and NYSDOH
For more information please or order by calling 0845 408 2084.
Additional information about the science behind the testing process visit our partner at www.promega.com.
DNA Testing Costs
StemElite™ ID............................ £119 per sample
PowerPlex® 16 ............................ £119 per sample
DNA Isolation ............................ £19 per sample - Using Promega Wizard® SV Genomic Extraction
or call 0845 408 2084 to Order Now!
Sample Shipping Guidelines
- Wash the centrifuged cell pellet with phosphate buffered saline (PBS) at least three times.
- Prepare the cell pellet containing 1x106 - 5x106 cells OR purified DNA - 10 - 100 μl at a minimum of 2 ng/μl, if performing your own extraction. Place the dried cell pellet in a small culture tube or vial.
- Send samples to our laboratory by overnight courier with adequate dry ice so that samples remain frozen during shipment:
Shipping Address:
DNA Diagnostics Centre
Britannia House
11 Glenthorne Road
London, W6 0LH
Click here to download shipping instructions PDF
Ordering Process
To purchase and begin the Cell Line Identification process follow these simple steps:
- Download a PDF of the order form here or call DDC at 0845 408 2084 to commence.
- Complete Order Form and fax to 020 8563 1858
- Carefully follow shipping guidelines above, and send samples to:
DNA Diagnostics Centre
Britannia House
11 Glenthorne Road
London, W6 0LH
- You will be contacted upon sample arrival and receive a completion date
- Once the testing is concluded, your results will be sent via your preferred method(s)
Click here to download ordering process PDF
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